Table 3.
Summary of epitopes identified from HSV-2 proteins in a healthy human HLA-A2+ donor (1185) with % sequence homology to HSV-1. The set threshold for high affinity binding peptides was given an AI of ≥1.5, and for intermediate affinity peptides was given an AI of 1.25–1.5.
| HLA-A2 Restricted Epitope | Peptide Sequence | % SEQUENCE Homology to HSV-1 a | Affinity Index (AI) b | Mean SFU/million PBL |
|---|---|---|---|---|
| ICP27(314-322) | TLVAHGPSL | 100 | 1.70 | 377.5 |
| ICP27(428-436) | FVLVILARL | 88.8% | 1.42 | 107.5 |
| VP22(173-181) | AKKLHFSTA | 88.8% | 1.28 | 152.5 |
| VP13/14(242-250) | FASPADPKV | 66.7% | 1.61 | 132.5 |
a Alignments were performed using genebank HSV-1 ICP27 accession AB235845.1, HSV-1 VP22 accession NP_044651, HSV-1 VP13/14 accession NP_044649. HSV-2 alignments were performed with ICP27 accession NP_044525, VP22 accession NP_044519, VP13/14 accession ABS84906; b Assessed by T2 stabilization. Affinity index > 1.5 high affinity, 1.25-1.49 was considered as an intermediate affinity peptide.