Figure 9.

Gene delivery of ChABC leads to increased serotonergic innervation following spinal contusion. A–D, Serotonergic (5-HT) fiber density is significantly enhanced in the ventral horn of the lumbar spinal cord following treatment with LV-ChABC (C) compared with contusion only (A) and LV-GFP (B; quantified in D; n = 4 per group; p < 0.05, 1-way ANOVA, Tukey's post hoc). E–G, C-4-S immunoreactivity is abundant in the lumbar cord of LV-ChABC-treated animals (F), but absent in both LV-GFP and contusion-only animals (E; quantified in G; n = 4 per group; p < 0.001, 1-way ANOVA, Tukey's post hoc); boxes in A and E show regions where intensity measurements were quantified. Scale bar: (in C) A–C, 250 μm; (in F) E, F, 500 μm.