Figure 2.

Schematic representation of the genomic organization of alternative splice variants of the human NKCC1 gene. The genomic organization of the human NKCC1 gene (isoform NKCC1a) is shown at the top of the figure, along with horizontal arrows indicating transcriptional orientation. The continuous line indicates genomic sequence, on which known exons of NKCC1 are shown as rectangles and numbered. Novel exons (exon 2a located in intron 2, 4a in intron 4, and 21a in intron 21) are shown as small rectangles. An oval represents the location of the stop codon for the ORF starting with NKCC1 exon 1; the open oval indicates a premature termination codon (PTC); the closed oval indicates the normal stop codon. The predicted MW of each protein isoform, translated from the first start codon within NKCC1 exon 1 up to the normal stop codon or the PTC, was calculated using the Compute pI/Mw tool (http://www.expasy.org/tools/pi_tool.html) and shown under the column MW. Variants used in transfection experiments are marked with asterisks.