Figure 13.

Knockdown of DCX causes increased interneuron branching and blocks SDF1 reduction of branching. A, Frames from Movie 9. A, B, Knock down of DCX by DCX-shRNA causes interneurons to branch more frequently than control cells. This increase in branching can be rescued by cotransfecting with DCX or DCX-S47A expression plasmids. In mock-transfected (dsRed) control conditions, SDF1 reduces branching. Under DCX knockdown conditions, SDF1 branching reduction is blocked. Under DCX rescue conditions, SDF1 reduces branching relative to control. When rescue is performed by DCX-S47A, a PKA-insensitive mutant form of DCX, SDF1 causes an even greater reduction in branching (±SEM; p < 0.001, analysis of deviance, ***p < 0.001, **p < 0.01, *p < 0.05, post hoc tests). At least 23 cells per condition, three independent experiments. Scale bar, 10 μm.