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ITC thermograms of ubiquitin binding to UIM-SH3 of STAM. (a) Thermogram of ubiquitin binding to UIM-SH3 revealing a KD of 273 ± 16 µM. (b) Thermogram of ubiquitin binding to the SH3 domain of STAM revealing a KD of 62 ± 7 µM. (c) Thermogram of Lys63- linked diubiquitin binding to UIM-SH3 revealing a KD of 54 ± 21 µM.
