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. Author manuscript; available in PMC: 2014 Nov 5.
Published in final edited form as: Biochemistry. 2013 Oct 23;52(44):7818–7829. doi: 10.1021/bi401106b

Figure 7.

Figure 7

DUB activity assay by monitoring diubiquitin cleavage. SDS-PAGE gel comparing the activity of the catalytic domain of AMSH alone and in the presence of the SH3 domain and UIM-SH3 of STAM. Only the lane with UIM-SH3 reveals activation. The asterisk indicates ubiquitin contamination in the diubiquitin purification.