Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Catalytic activation of AMSH in presence of UIM-SH3. (a) Domain diagram of the minimal AMSH and STAM proteins, indicating the locations of the introduced mutations. (b) SDS-PAGE investigating the effects of mutants on the catalytic activation of AMSH. Only in the presence of the wild-type enzyme and wild-type UIM-SH3 is the activity of AMSH enhanced, indicated by complete disappearance of the diubiquitin substrate (black arrow). All lanes have Lys63-linked diubiquitin.
