Table 2.
Thermodynamic Parameters Deduced from ITC Data
| Protein | Titrant | KD (µM) | ΔH (kcal.mol−1) | ΔS (cal.mol−1.K−1) | N |
|---|---|---|---|---|---|
| AMSH | Ub | 19 ± 3 | 19.3 ± 7.8 | 86.4 | 1 |
| AMSH | DiUb | 19 ± 4 | 13.7 ± 1.8 | 67.7 | 1 |
| AMSHF320A | Ub | 81 ± 15 | 2.6 ± 0.4 | 27.5 | 1 |
| AMSH | SH3 | 1.4 ± 0.04 | −15.1 ± 0.1 | −23.7 | 1 |
| AMSH | UIM-SH3 | 1.9 ± 0.1 | −15.8 ± 0.1 | −26.9 | 1 |
| AMSHK238A | SH3 | NAa | NAa | NAa | NAa |
| AMSHK238T | SH3 | NAa | NAa | NAa | NAa |
| SH3 | Ub | 62 ± 7 | −4.1 ± 0.2 | 5.3 | 1 |
| UIM-SH3 | Ub | 273 ± 16 | −18.2 ± 1.0 | −44.7 | 2 |
| UIM-SH3 | DiUb | 54 ± 21 | −13.0 ± 6.6 | −23.9 | 1 |
a
No observed binding at 50 µM of enzyme and 1 mM of UIM-SH3