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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: Chem Biol. 2008 Aug 25;15(8):854–862. doi: 10.1016/j.chembiol.2008.06.008

Figure 1.

Figure 1

Coomassie stained 10% PAGE SDS gel (A) and western blot (B) analysis of IMAC-purified, recombinant hexa-histidine-tagged human MGL (hMGL). (C) Best-fit, one-site plots of the concentration-dependent inhibition by AM6701, AM6702, and NAM of hMGL and designated hMGL cysteine mutants to hydrolyze AHMMCE. The inset highlights the biphasic nature of the hMGL C249A mutant by NAM as a two-site plot. (D) IC50 values for inhibition of soluble crude rMGL, hMGL, and MGL prepared from COS cells and rat cerebellum by AM6701, AM6702, and NAM.

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