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Relative Content of NAM-modified, Cysteine-containing Peptides Identified by MS in Tryptic Digest of NAM-treated hMGL
| hMGL cysteine residues | m/z | Relative Alkylation NAM:hMGL ratio |
|
|---|---|---|---|
| 8.3:1 | 33:1 | ||
| Cys39 | 3144.62; 3300.72 | 0% | 100%(a) |
| Cys208 | 2007.05 | 0% | 0%(a) |
| Cys215 | 2158.28 | 26%(b) | 22%(b) |
| Cys249 | 2445.38; 3534.97 | 74%(b) | 78%(b) |
percent alkylation of Cys39 relative to Cys208
percent alkylation of Cys215 relative to Cys249
