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. Author manuscript; available in PMC: 2015 Mar 3.
Published in final edited form as: FEBS Lett. 2014 Jan 23;588(5):752–756. doi: 10.1016/j.febslet.2014.01.021

Figure 2.

Figure 2

Steady-state kinetic analysis of LodA. A. Initial rates were determined at different concentrations of lysine in the presence of a fixed concentration of 252 (○), 129 (♦), 54 (▼), 11 (▲) or 5 (●) µM O2. B. Double reciprocal plot of the data shown in panel A. C. Secondary plots of the y-intercept (1/apparent kcat) from panel B versus 1/[O2]. Data are fit by eq 3. D. Secondary plots of the y-intercept/slope (1/apparent Klysine) from panel B versus 1/[O2]. Data are fit by eq 4. E. Secondary plots of the y-intercept (1/apparent kcat) from plots of v/E versus [O2] against 1/[lysine]. Data are fit by eq 3. F. Secondary plots of the y-intercept/slope (1/apparent KO2) from plots of v/E versus [O2] against 1/[lysine]. Data are fit by eq 4. Error bars are indicated in the secondary plots (C–F).