Table 2. Summary of the F2 offspring from mating shown in Figure1a.
|
F2 offspring |
|||
|---|---|---|---|
| Genotypes of breeding pairs | Floxed allele | Cre | No. offspring (%) |
| F1 male: Rosa26+/stop-lacZ:: CaMKIIα-Cre+/Cre | +/stop-lacZ | + | 1 (0.9) |
| +/stop-lacZ | Cre | 0 (0.0) | |
| +/lacZ | + | 31 (26.5) | |
| +/lacZ | Cre | 33 (28.2) | |
| Female: wild type | +/+ | + | 31 (26.5) |
| +/+ | Cre | 21 (17.9) | |
| Total | 117 (100) | ||
The lacZ and stop-lacZ alleles were determined by X-gal staining. The CaMKIIα-driven Cre recombinase deleted the stop sequence and the resulting lacZ embryos were X-gal positive in the entire body (Figure 1e).