Figure 4.

The effect of HDAC inhibitors on the levels of histone acetylation. (a) Western blot analysis of protein extracts from 5 dpf control larvae and larvae treated with HDAC inhibitors for 2 days starting at 3 dpf. HDAC inhibitors induced the accumulation of acetylated (Ace) H3 and H4. Histones H3 and H4 were included as controls. (b) Confocal images of neuromasts from a 5 dpf control larva and from 5 dpf larvae treated with TSA, VPA or MS-275. Larvae were treated with 0.1 μM TSA, 100 μM VPA or 10 μM MS-275 for 2 days starting at 3 dpf and were stained (red) by antibodies against Ace histone H3 and Ace histone H4. The levels of Ace histone H3 and Ace histone H4 are increased in the presence of HDAC inhibitors compared with the controls (CON). HCs were labeled with antibodies against Myosin VI (green) and nuclei were stained with DAPI (blue). Scale bar=10 μm.