Table 1. A list of primers and reaction conditions for RT-PCR analysis.

Gene (symbol)	Sequence (5′→3′)	Tm (°C)	Species	Product size (bp)
IL-1β
	F—TGAAGCAGCTATGGCAACTG	55	Rat	199
	R—TGCCTTCCTGAAGCTCTTGT
TNFα
	F—TGCCTCAGCCTCTTCTCATT	55.4	Rat	367
	R—TGTGGGTGAGGAGCACATAG
NOS2
	F—CACCTTGGAGTTCACCCAGT	60	Rat	170
	R—ACCACTCGTACTTGGGATGC
ICAM-1
	F—CAGGGTGCTTTCCTCAAAAG	55	Rat	249
	R—GGGCATGAGACTCCATTGTT
MMP-9
	F—GTCTTCCCCTTCGTCTTCCT	55	Rat	249
	R—AGGGGAGTCCTCGTGGTAGT
ITGa5
	F—AGGTGACGGGACTCAACAAC	60	Rat	151
	R—GGGCATTTCAGGACTTGTGT
SCIN
	F—ACCAGAGACGAGCTGACGAT	60	Rat	101
	R—GGCTCTTTGCCTTGAGACAC
Rac1
	F—TTTGAAAATGTCCGTGCAAA	60	Rat	306
	R—CAGCAGGCATTTTCTCTTCC
scin
	F—ACCAGAGACGAGCTGACGAT	60	Rat	101
	R—GGCTCTTTGCCTTGAGACAC
β-Actin
	F—TGTTGGCATAGAGGTCTTTACGG	60	Rat	278
	R—TGAGAGGGAAATCGTGCGTG
GAPDH
	F—ACCACAGTCCATGCCATCAC	60	Rat	452
	R—TCCACCACCCTGTTGCTGTA
IL-1 β
	F—CCGATGGGTTGTACCTTGTC	60	Mouse	284
	R—CGGACTCCGCAAAGTCTAAG
TNFα
	F—GACCTTCCAGGATGAGGACA	60	Mouse	283
	R—AGGCCACAGGTATTTTGTCG
β-actin
	F—TGTTACCAACTGGGACGACA	60	Mouse	391
	R—TCTCAGCTGTGGTGGTGAAG

Abbreviations: IL, interleukin; RT-PCR, Reverse transcription polymerase chain reaction; TNF, tumor necrosis factor.