Fig. 6.

(a) Cell viability assay of rat liver cells cultured for seven days in the perfused multiwell. The image shows multiple channels in a scaffold. Live cells are stained green with calcein AM while dead cells are stained red with ethidium homodimer-1. The areas occupied by the cells are bright green and areas without cells appear dark green or black. (b) Immunostaining of rat liver cells cultured in the perfused multiwell 7 days post seeding demonstrating retention of the hepatocyte-specific functional marker albumin (green). Staining was preformed with rat albumin antibody. This image shows a single channel with its boundary highlighted by a white dash line. (c) Image of rat hepatocytes and sinusoidal endothelial cells cultured in the perfused multiwell plate day 13 post seeding. Nuclei are stained blue with DRAQ 5, non-parenchymal cells express green fluorescent protein (GFP), and the functional marker for sinusoidal endothelial cells (SE-1) is stained red.