Skip to main content
. 2014 Mar 12;2014:136419. doi: 10.1155/2014/136419

Table 2.

Best-performing plant-based platforms for the production of four selected recombinant pharmaceutical proteins.

Plant host Plant organ Recombinant protein Expression system Highest expression level Plants/g recombinant protein Reference
Tobacco (Nicotiana tabacum) Leaves hGAD65mut Transgenic 0.14 mg/g LFW 193 [23]
NVCP Transgenic 0.2% TSP ND [41]
IL6_KDEL Transgenic 0.11 mg/g LFW 1119 [108]
2G12/2G12_KDEL Transient (binary vector) 0.1 mg/g LFW 1133 [78]
Seeds hGAD67/65mut Transgenic 0.4 mg/g DSW 11250 [26]
IL6_KDEL Transgenic 0.3 mg/g DSW 11667 [108]
2G12_KDEL/ELP Transgenic 1.0% TSP ND [80]
Nicotiana benthamiana Leaves hGAD65mut Transient (MagnICON vectors) 0.1 mg/g LFW 23451 [23]
NVCP (VP1 and VP2) Transient (binary vector) 1 mg/g FLW 2333 [57]
IL6_KDEL Transient (MagnICON vectors) 7.8% TSP ND [108]
2G12_KDEL Transient (viral vector) 0.12 mg/g LFW 22693 [79]
Arabidopsis thaliana Seeds hGAD67/65mut Transgenic 4.5 mg/g DSW 3308 [26]
2G12 Transgenic 3.6 mg/g DSW 3385 [75]
Leaves 2G12 Transgenic 0.2% TSP ND [73]
Maize (Zea mays) Seeds 2G12 Transgenic >0.1 mg/g DSW ND [76]
Lettuce (Lactuca sativa) Leaves NVCP Transient (viral vector) 0.2 mg/g LFW ND [56]
Petunia (Petunia hybrida) Seeds hGAD67/65mut Transgenic 0.2 mg/g DSW ND [26]
Carrot (Daucus carota) Taproots hGAD65 Transgenic 0.01% TSP ND [22]
Chlamydomonas reinhardtii   hGAD65 Transgenic 0.3% TSP ND [20]
Potato plant (Lycopersicon esculentum) Fruits NVCP Transgenic 0.16 mg/g fruit weight 40.6 [53]
Tomato (Solanum tuberosum) Tubers NVCP Transgenic 0.12 mg/g tuber weight ND [53]

The highest expression levels are reported as mass of recombinant protein per unit of biomass (LFW: leaves, fresh weight; DSW: dry seed weight) unless these values were not available, in which case percentage total soluble protein (%TSP) is used instead. The recombinant protein productivity values were calculated by considering the seed or leaf biomass yield per plant (1[115]; 2[59]; 3 [116]; 4 Mississippi State University website: http://msucares.com/crops/comhort/yield.html). ND: not determined (values were not calculated because of the absence of productivity data or the expression data were reported as %TSP).