Table 2.

Summary of analysis for growth cone area, primary neurites emanating from DRG neurons, and the number of branches from primary neurites under various pharmacological conditions. Note that analysis of neurite length was the most reliable analysis possible for dorsal horn neurons. The data show that those morphological indices are unchanged by KA receptor activation or blockade except for the mean number of branches on the DRG primary neurite in Sym2081. All values are expressed as mean ± SEM and P-values were calculated by one way ANOVA followed by Dunnett's multiple comparison post hoc tests.

DRG neurons
Agonist	250 nM KA	10 μM KA	100 μM KA	Control
Growth cone area, μm2	35.7±3.2	33.3±4.3	31±3	38±3.4
p>0.05 by ANOVA	n=36	n=47	n=41	n=72
And Dunnett's test
Mean number of branches on the primary neurite	3±0.4	3.8±0.33	3.4±0.42	4.2±0.4
p>0.05 by ANOVA	n=30	n=44	n=38	n=61
And Dunnett's test
Number of primary neurites	2.05±0.18	2.52±0.17	2.18±0.12	2.5±0.18
p>0.05 by ANOVA	n=37	n=54	n=45	n=48
And Dunnett's test
Antagonists	Sym2081	CNQX	JSTX	PhTX	Control
Growth cone area, μm2	46±3.4	40±4.8	48±4.7	48.5±5	38±3.4
p>0.05 by ANOVA	n=74	n=26	n=54	n=41	n=72
And Dunnett's test
Mean number of branches on the primary neurite	6±0.4	3. ±0.4	5±0.45	5±0.5	4.2±0.42
P<0.05 by ANOVA	n=77	n=43	n=67	n=51	n=61
And Dunnett's test	*p<0.05	p>0.05	p>0.05	p>0.05
Number of primary neurites	2.2±0.11	2.3±0.14	2.4±0.14	2.2±0.16	2.2±0.13
p>0.05 by ANOVA	n=88	n=42	n=69	n=52	n=58
And Dunnett's test

Dorsal horn neurons
	10 μM KA	Sym2081	CNQX	JSTX	PhTX	Control
Neurite length μm	165±24	175±18	167±24	173±16	175±18	166±18
p>0.05 by ANOVA	n=22	n=39	n=27	n=49	n=33	n=69
And Dunnett's test