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. Author manuscript; available in PMC: 2014 Apr 2.
Published in final edited form as: J Autoimmun. 2013 May 14;44:71–81. doi: 10.1016/j.jaut.2013.04.001

Fig. 5.

Fig. 5

B7-H4Ig treatment decreases the number of effector CD4+ T cells and increases the number of Tregs in the CNS. SJL-FoxP3/GFP mice (n = 5/group) were primed with PLP139–151/CFA, and treated with Control-Ig or hB7-H4Ig beginning during disease remission. Following the final treatment mice were perfused, and histological analysis of the lumbar spinal cord, cerebellum, and the spleen. Tissues were frozen in OCT, and 8 μm sections from the lumbar spinal cord were stained for (A) PLP (green) and nuclei (DAPI), and (B) CD4 (red), FoxP3 (green), and nuclei (DAPI). (C) For the sections taken from the cerebellum, similar analysis was completed, and a decrease it the number of CD4+ T cells was seem with a coordinate increase in the number of FoxP3+ cells within the lesions of hB7-H4Ig treated mice (white arrow). (D) Spleen sections were analyzed for the presence of CD4+ cells (red), FoxP3+ cells (green), and nuclei (DAPI).