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. 2014 Mar 18;124(4):1745–1756. doi: 10.1172/JCI44071

Figure 4. hMDSPCs integrate into the microenvironment of the regenerated nerve and generate myelinating Schwann cells.

Figure 4

Representative images are of immunostained longitudinal sections of hind limbs, including the regenerating sciatic nerve, 4 weeks after injury and after hMDSPC transplantation. Low- and high-magnification images show immunostaining for neurons (NF, red), myelinating Schwann cells (CNPase, green), human donor cell nuclei (PCNA, blue), and DAPI (white) to identify all nuclei. Donor PCNA+ hMDSPCs were detected inside the regenerated nerve in immediate proximity to the myelinated axons and expressed NF and CNPase at the middle (A) and distal (B) regions of the regenerating nerve. Panels on the right show higher-magnification images of the chosen area (dashed boxes), highlighting the colocalization. Engraftment and colocalization were determined by screening more than 10,000 donor cells from 4 independent transplanted hMDSPC populations within 982 sections of 122 tissues obtained from 12 mice. Original magnification, ×20 (A and B) and ×100 (dashed boxes).