Figure 2. Compromising insulin signaling in osteoblasts worsens glucose intolerance in mice fed a HFD.

(A) qPCR analysis of the expression of Insr in bones, livers, muscles, and WAT of Insr^fl/+ and Col1a1-Insr^+/– mice (n = 6). (B) Body weight and (C) gonadal fat pad weight in Insr^fl/+ and Col1a1-Insr^+/– mice fed a normal diet or HFD (n = 8). (D) Random glucose levels in Insr^fl/+ and Col1a1-Insr^+/– mice fed a normal diet or HFD (n = 8). (E) Random insulin levels in Insr^fl/+ and Col1a1-Insr^+/– mice fed a normal diet or HFD (n = 8). (F) GTT and (G) ITT in Insr^fl/+ and Col1a1-Insr^+/– mice fed a normal diet or HFD (n = 8). (H) Western blot analysis of the phosphorylation levels of INSR and AKT in muscles of WT and Col1a1-INSR^Tg mice fed a normal diet or a HFD. All Western blot experiments were repeated at least 3 times.*P < 0.05.