Figure 3. Insulin resistance develops in bones of mice fed a HFD.

(A) Western blot analysis of the phosphorylation levels of INSR, AKT, and GSK3β in bones, livers, muscles, and WAT of WT mice fed a normal diet or a HFD. (B) Western blot analysis of phosphorylation levels of INSR and AKT in bones of Insr^fl/+ and Col1a1-Insr^+/– mice fed a normal diet or a HFD. (C) Western blot analysis of the phosphorylation levels of INSR and AKT in bones of WT and Col1a1-INSR^Tg mice fed a normal diet or a HFD. All Western blot experiments were repeated at least 3 times. *P < 0.05.