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. 2014 Mar 10;124(4):1672–1684. doi: 10.1172/JCI63139

Figure 8. CDK4 expression is suppressed, and FOXO1 and RAG1 expression is elevated in several subtypes of human B cell lymphomas.

Figure 8

(A) 125 cases of randomly collected patient B cell lymphoma samples from The Ohio State University Department of Pathology and US Biomax Inc., along with 10 normal LNs, were stained with antibodies specific to CDK4 (top panels), p-FOXO1-S329 (second panels), FOXO1 (third panels), or RAG1 (bottom panels). Original magnification, ×400. (B) For statistical analysis, the samples were graded on a scale of staining scores from 1 (lowest) to 5 (maximum), based on signal intensity for CDK4, p-FOXO1-S329, FOXO1, or RAG1. Approximately 90% of B cell lymphoma samples (111 of 125) had low or nondetectable levels of CDK4 (stages 1 and 2), and nearly 70% (87 of 125) of B cell lymphoma samples had low levels of p-FOXO1-S329 (stages 1 and 2). Accordingly, approximately 70% of these tumors (86 of 125) had elevated levels of total FOXO1 (stages 4 and 5), and approximately 64% (80 of 125) had elevated levels of RAG1 (stages 4 and 5). Among the lymphomas with elevated levels of FOXO1, more than 70% (61 of 86) had elevated levels of RAG1.