Table 2. Summary of somatic genetic mutations and rearrangements detected by next-generation sequencing assay and results of MCL1 IHC staining.

Patient no.	KRAS mutation	GNAS mutation	Other mutations	MCL1 IHC staining	No. of genetic alterations
1	G12D	R201H		Moderate	2
2	A59T		hMLH1 micro satellite instability	Moderate	1a
3	G12V	R201C	Amplification of MCL1 and JUN	Weak	4
4	G12D			Weak	1
5	G12A		Amplification of ZNF703	Moderate	2
6			None detected	Moderate to strong	0
7			None detected	Moderate to strong	0
8	G12D	R201H	Amplification of MCL1 and JUN	Moderate	4
9			Amplification of MCL1, MYC, JUN and NFKBIA	Moderate	4
10	G12D	R201C		Moderate to strong	2

Abbreviation: IHC, immunohistochemical.

^aThe hMLH1 microsatellite instability was not counted.