Figure 3. TALEN generated mutations in Scn8a and off-site mutations in two sodium channel paralogs.

The 327 bp fragment shown in Figure 2A was amplified from the genomic DNA of 62 potential founder mice and sequenced. A. Sequence chromatogram from a founder mouse carrying Scn8a^tm1768DMm. The 9 nucleotide substitutions from the targeting construct in Figure 1B were detected in heterozygous state (yellow). B. Genomic sequence of the Scn8a locus from 24 founder mice carrying 13 different indels. C, D. Genomic sequence from 7 founders carrying 5 different indels in the cardiac sodium channel Scn5a and two indels in the muscle sodium channel channel Scn4a. The wildtype sequences are shown in Figure 1C. The number of independent alleles observed is indicated at the right. *, homozygote.