Isolation and transduction of progenitors. (A): CD34+lin– cells were sorted to obtain common myeloid progenitor (CMP), megakaryocytic–erythroid progenitor (MEP), and granulocytic monocytic progenitor (GMP). (B): Relative colony-forming unit output from CMP, MEP, GMP, and total CD34+ cells (500 cells per population plated). (C): Relative B-lymphoid output in lymphoid cultures initiated with populations shown. (D): Lentiviral F36VMpl vector. Abbreviations: CFU, colony-forming unit; CMP, common myeloid progenitor; F36VMpl, fusion protein; GFP, green fluorescent protein; GMP, granulocytic monocytic progenitor; HSC: hematopoietic stem cell; MEP, megakaryocytic erythroid progenitor; MSCV LTR, murine stem cell leukemia virus long-term repeat promoter; PGK, human phosphoglycerate kinase promoter.