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. Author manuscript; available in PMC: 2014 Apr 2.

Published in final edited form as: Stem Cells. 2012 Apr;30(4):697–708. doi: 10.1002/stem.1046

Dimerization in common myeloid progenitor (CMP) induces expansion of CD45+, CD34+, and erythroid cells and enhances cell cycling and survival. Transduced CMP cultured ± chemical inducer of dimerization without growth factors (starting cell number = 7,000–53,000 cells per arm, n = 5 independent experiments, equal cell numbers were used for each arm in a given experiment) were analyzed for (A) cell output, (B) immunophenotype (day7), as well as (C) cell cycle and apoptosis (day 7). (D): Giemsa-Wright staining ( ×10 magnification; red arrows, erythrocytes; green arrows, erythroid progenitors) and (E) flow cytometry for enucleated erythrocytes in dimerized cultures (day 35). Abbreviations: CID, chemical inducer of dimerization; GFP, green fluorescent protein; Gly A, glycophorin A. , F36VMpl+CID; , F36VMpl, no CID; , F36V+CID.

Inline graphic — Dimerization in common myeloid progenitor (CMP) induces expansion of CD45+, CD34+, and erythroid cells and enhances cell cycling and survival. Transduced CMP cultured ± chemical inducer of dimerization without growth factors (starting cell number = 7,000–53,000 cells per arm, n = 5 independent experiments, equal cell numbers were used for each arm in a given experiment) were analyzed for (A) cell output, (B) immunophenotype (day7), as well as (C) cell cycle and apoptosis (day 7). (D): Giemsa-Wright staining ( ×10 magnification; red arrows, erythrocytes; green arrows, erythroid progenitors) and (E) flow cytometry for enucleated erythrocytes in dimerized cultures (day 35). Abbreviations: CID, chemical inducer of dimerization; GFP, green fluorescent protein; Gly A, glycophorin A. , F36VMpl+CID; , F36VMpl, no CID; , F36V+CID.