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. Author manuscript; available in PMC: 2015 Mar 26.
Published in final edited form as: Vaccine. 2014 Feb 7;32(15):1670–1677. doi: 10.1016/j.vaccine.2014.01.063

Figure 1.

Figure 1

pmhyGENIE-3 and pmhyGENIE-3-ΔPB show equivalent in transfection efficiency and protein expression. HEK293 (1 × 106) cells were transfected with 2 μg of each plasmid, (A) pmhyGENIE-3-eGFP and pmhyGENIE-3-ΔPB-eGFP or (B) pmhyGENIE-3-GL3 and pmhyGENIE-3-ΔPB-GL3. Cells were monitored over time for percent cells with eGFP signal or luciferase activity as described in the Methods section. Data are shown for 48 h post-transfection and represent mean + S.E.M. (N = 3), means were compared using a student’s t-test with significance at *P< 0.05. In both cases, no significant differences were found. Results are representative of two independent experiments.