Figure 5.

Light stress in Rpe65^rd12 mice. (A) Reverse phase HPLC chromatograms with UV-visible detection at 430 and 490 nm. The bisretinoids A2E, A2-DHP-PE, and atRAL dimer–PE are detected in eyes of Abca4^−/− mice (age 6 months; four eyes pooled/sample) but are not detected in Rpe65^rd12 mice (age 8 months; eight eyes pooled/sample). (B) Measurement of ONL in light-exposed (right) and control (left) eyes of Rpe65^rd12 mice, age 5 months. Thicknesses are plotted as distance from ONH. C57BL/6J mice (6 months) served as wild type. Values are mean ± SEM of numbers of eyes presented in parentheses.