Figure 4.

H. pylori-induced TRAIL sensitivity is dependent on Bid processing. (a) AGS cells were transfected with Bcl-2, followed by incubation with H. pylori and subsequently treated with TRAIL. Cell apoptosis was measured by cell death enzyme-linked immunosorbent assay (ELISA). Values represent means±S.D. of three independent experiments (*P<0.05). (b) AGS cells were incubated with or without H. pylori followed by treatment with TRAIL at the indicated times. The expression of full-length and truncated Bid (22 and 15 kDa, respectively) was detected by western blotting. Membranes were also blotted with anti-β-actin mAb as a loading control. (c) Cells were transfected with scramble or Bid siRNA, followed by incubation with H. pylori and subsequently treated with TRAIL. Cell apoptosis was measured by cell death ELISA. Values represent means±S.D. of three independent experiments (*P<0.05)