Figure 5.
H. pylori downregulates FLIPS expression and enhances the TRAIL-induced DISC assembly. (a) AGS cells were incubated with increasing amounts of H. pylori (MOI 25, 50, 100, 200) for 12 h and subsequently treated with TRAIL. Cell lysates were analyzed by western blotting to detect the activation level of caspase-8. Actin expression was used as a protein loading control. (b) Cells were co-cultured with H. pylori for 12 h, stimulated with 1 μg/ml FLAG-TRAIL combined with 2 μg/ml anti-FLAG antibody or normal mouse IgG for 1 h, and cell lysates were precipitated with protein A agarose. Isolated receptor complexes (IP) were assessed for caspase-8, FLIPS, FADD, and DR5 by western blotting