Figure 6.

H. pylori-induced sensitization to TRAIL-induced apoptosis is via c-FLIP_S downregulation. (a) AGS cells were transfected with FLIP_S and pCMV mock control, followed by incubation with H. pylori and subsequently treated with TRAIL. Cell apoptosis was measured by cell death enzyme-linked immunosorbent assay. Values represent means±S.D. of three independent experiments (*P<0.05). (b) Cells were pretreated with 2 μM MG132, a proteosome inhibitor, for 30 min before incubation with H. pylori (MOI 50,100, 200). After co-culture for 12 h, c-FLIP_S was determined by western blotting. The expression of actin serves as a loading control