Figure 3.

The miR-23a/b and miR-27a/b suppress Apaf-1 protein expression. (a) HEK-293T cells were transfected with luciferase reporter plasmids carrying wild-type (WT) or mutant (Mut) Apaf-1 3′-UTRs. The luciferase activities were examined in the presence of pre-miR-23a/b (left) or pre-miR-27a/b (right) (n=5, unpaired t-test, NS, not significant, **P<0.01 and ***P<0.001). (b) Apaf-1 protein level in HEK-293T cells 48 h after transfection with pre-miR-23a/b or pre-miR-27a/b (n=5, unpaired t-test, *P<0.05 and **P<0.01)