Figure 1.

The p42 specifically interacts with p85 regulatory subunit of PI3K. (a) HEK 293T cells were transfected as indicated. Cell lysates were immunoprecipitated with anti-Myc antibody, and endogenous p85 protein was determined by immunoblotting with the anti-p85 antibody. (b) Transfected cells were subjected to immunoprecipitation and immunoblotting as indicated (left). To detect endogenous interaction between p85 and p42 or p48, 293T cell lysates were immunoprecipitated with anti-p85 antibody and immunoblotting with anti-Ebp1 or anti-N-Ebp1 antibody (right). (c) Schematic diagram of the Ebp1 fragments. (d) Flag-p85 and GFP-Ebp1 co-transfected cells were immunoprecipitated with anti-Flag and immunoblotted as indicated. (e) The schematic diagram of various domains of the p85 subunit (left), and binding analysis between GFP-p42 and Flag-p85 fragments (right)