Figure 2.

Recruitment and function of GrzM^−/− NK cells is altered in the liver after L. monocytogenes infection. (a and b) Age-matched female WT and GrzM^−/− mice were infected i.v. with 10⁴ CFU of L. monocytogenes. At indicated time points after infection, mice were killed, livers (a) and spleens (b) were harvested, lymphocytes isolated and stained with mAbs reactive with NK1.1 and CD3 (shown are absolute numbers of NK cells), with (c) NK1.1, CD3, CD27 and CD11b, with Ly6G⁻/CD11b⁺/F4/80⁺ (d), CD4 (e), CD8 (f) or NK1.1, CD3 and IFN-γ (g) and analysed by flow cytometry. Results are pooled from six independent experiments, total n=5–8