Figure 6.

GrzM does not process MIP-1α. (a) Human recombinant full-length GST-tagged MIP-1α (sequence start mature MIP-1α: bold letters) was generated. The protein contains a GrzM consensus cleavage site in the polylinker region between GST and the start of full-length MIP-1α, as well as a potential cleavage site for GrzM in the full-length MIP-1α sequence. (b) This product was then incubated with low concentrations of human recombinant GrzM or a Serine/Alanine (S/A) mutant. The resulting products were used for immunoblotting (c) N-terminal sequencing and (d) mass spectrometry