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. 2014 Mar 6;5(3):e1110. doi: 10.1038/cddis.2014.83

Figure 2.

Figure 2

In vitro expression levels of mutant FAS variants. (a) JK cells transfected with wild-type or mutant FAS variant pKEX constructs. Flow cytometry showing endogenous (shaded) and exogenous (solid) surface FAS expression levels 48 h after transfection. Figure shows a representative of three independent experiments. (b) HEK-293T cells transfected with wild-type or mutant FAS variant pKEX constructs. Western blot of (panel 1) cellular protein extracts and (panel 2) conditioned medium protein extracts. NTC, non-transfected control