Figure 2.

Hematopoietic stem/progenitor cell defects in Cav-1^−/− mice. (a) Representative staining profiles for BM HSCs and progenitor populations. (b) Cell numbers of LSKs, LT-HSCs, ST-HSCs and MPPs in the BM from 2-mo- and 12-mo-old (c) mice. (d) Representative staining profiles for CLPs (Lin⁻Sca-1^lowc-Kit^lowCD127⁺) in the BM. (e) Cell numbers of CLPs in the BM from 2-mo- and 12-mo-old mice. (f) Representative staining profiles for myeloid progenitors. L⁻S⁻K⁺ cells were subdivided into CMPs (Lin⁻Sca-1⁻c-Kit⁻CD34⁺CD16/CD32⁻), GMPs (Lin⁻Sca-1⁻c-Kit⁻CD34⁺CD16/CD32⁺) and MEPs (Lin⁻Sca-1⁻c-Kit⁻CD34⁻CD16/CD32⁻) as indicated. (g) Cell numbers of CMPs, GMPs and MEPs in the BM from 2-mo- and 12-mo-old (h) mice. n=5 mice per group. The experiment was repeated three times, and the results represent the mean±s.d. *P<0.05; **P<0.01