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. 2014 Mar 27;5(3):e1152. doi: 10.1038/cddis.2014.112

Figure 1.

Figure 1

HsPDF is overexpressed in human cancers. (a) HsPDF protein levels in cancer (black) and normal (white) cell lines as measured by flow cytometry. Median fluorescence intensity for 10 000 events was counted for each sample in triplicate (shown as mean±S.E.M.). Average sample median fluorescence intensity was significantly higher in cancer cells than the median fluorescence intensity in normal cells (*P<0.05) (b) HsPDF mRNA levels in cancer (black) and normal (white) cell lines as measured by qRT-PCR. Samples were normalized to B-actin and then compared with WI38, the lowest value. Mean mRNA fold HsPDF expression was significantly higher in cancer cells relative to normal cells (**P<0.01). (c) HsPDF protein levels in AML, CLL, multiple myeloma (MM), essential thrombocythemia (ET) and peripheral blood mononuclear cells (PBMCs). Median HsPDF AML fluorescence intensity=8800; median CLL fluorescence intensity=2400; median ET fluorescence intensity=3500; median PBMC fluorescence intensity=2100. (d) HsPDF mRNA relative to B-actin and then normalized to macrophages. Median AML message was 12-fold higher than macrophages (***P<0.001).