Figure 2.

HsPDF is regulated by c-Myc. (a) c-Myc is overexpressed in all cancer cell lines that also overexpress HsPDF. Normal cell lines did not have detectable levels of c-Myc. B-actin was used as a protein loading control. (b) Clockwise from top left: HsPDF protein expression doubles with c-Myc overexpression in P493 cells; HsPDF mRNA goes up eightfold with c-Myc overexpression; mitochondrial mass increase threefold as measured by mitochondrial DNA (ratio of complex III subunit 1/18sRNA). Western blot showing expression of c-Myc in the absence and presence of 1 μg/ml tetracycline. (c) HsPDF protein expression is abrogated in Ramos Burkitt's lymphoma cells when c-Myc is knocked down by small hairpin RNA (shRNA; left), western blot showing knockdown of c-Myc reduced HsPDF relative to B-actin (right). (d) HsPDF protein expression is abrogated when c-Myc is knocked down in Daudi Burkitt's lymphoma cells (right); western blot showing knockdown of c-Myc relative to B-actin. *P<0.05; **P<0.01; ***P<0.001