Figure 3.

Actinonin is selectively cytotoxic to c-Myc-positive cells. (a) Actinonin selectively kills c-Myc-positive P493 cells whether cells are proliferating (10% serum; IC50s Myc-positive=26 μM, Myc-negative≥100 μM, left panel) or not (0.25% serum; IC50s Myc-positive=27 μM, Myc-negative≥100 μM, right panel). (b) HsPDF levels do not change with serum deprivation or changes in the rate of proliferation (LS, low serum; HS, high serum). (c) Actinonin (ACT, 20 μM) selectively inhibits mitochondrial translation in c-Myc-positive cells (black bars), but not c-Myc-negative cells (white bars). Gel shows raw data and loading control. CAM (100 μM), a positive control, inhibits mitochondrial translation in both c-Myc-positive cells and c-Myc-negative cells. ***P<0.001