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. 2014 Mar 27;5(3):e1152. doi: 10.1038/cddis.2014.112

Figure 5.

Figure 5

Actinonin causes apoptosis through specific inhibition of HsPDF. (a) Recombinant HsPDF activity is inhibited by actinonin (100 nM) treatment for 2 h and is not prevented with pretreatment of CAM (100 μM). DMSO=vehicle control, Act=100 nM actinonin, CAM=100 μM CAM. (b) Actinonin (15 μM) causes caspase-3 activation that is prevented with pretreatment of CAM (100 μM) for 2 h in Ramos Burkitt's lymphoma cells. DMSO=vehicle control, CAM=100 μM CAM, staurosporine=2 μM, CAM+staurosporine=100 μM CAM 2 h before addition of 2 μM staurosporine. All caspase-3 activation assays were conducted 14 h after the addition of CAM or 12 h after the addition of actinonin or staurosporine. (c) Actinonin causes caspase-3 activation in Daudi Burkitt's lymphoma cells that is prevented with pretreatment with CAM (100 μM). (d) Actinonin (15 μM) causes caspase-3 activation that is prevented by pretreatment with tetracycline (10 μM) for 2 h. Caspase-3 activation assays were conducted at 12 h after addition of actinonin