Figure 4.

Aurora-C expression reduces BubR1 activation. (a) Aurora-C-GFP/WT-, KA-, or KD-transfected HeLa cells were treated with nocodazole for 4 h to turn on the spindle checkpoint. The kinetochore localization of BubR1 (red) was detected by IF analysis. DAPI is a DNA-specific dye. (b) Cells from (a) were used to examine BubR1 activation status using IB analysis. The expressions of Aurora-C-Myc/WT, KA, or KD are shown. GAPDH was used as a loading control. (c) HeLa cells transfected with vector control were treated with nocodazole and assessed for the phosphorylation status of BubR1 as described above. α-Tubulin was used as a loading control. The relative expression levels of individual proteins are displayed as ratios