Figure 3.
Portal hypertension enhanced gastric mucosal PUMA expression in CCl4-treated mice. (a) Histological analysis showed that CCl4 induced significant liver cirrhosis. The upper panel is H&E staining ( × 200), and the lower panel is Van Gieson staining ( × 200). (b) Portal hypertension significantly induced gastric mucosal injury (upper panel H&E staining, × 200) and upregulated PUMA (red) expression (lower panel immunofluorescence staining, × 200) in mice after CCl4 intraperitoneal injection for 12 weeks. Cell nuclei (blue) were counterstained by DAPI. (c) PUMA mRNA expression in the gastric mucosa was evaluated by real-time PCR in three different mice in each group. (d) Real-time PCR showed a relative PUMA mRNA fold change. The values are expressed as the means±S.D. (n=3 in each group) for real-time PCR. (e) PUMA protein expression in the gastric mucosa was determined by western blotting in three different mice. β-Actin was used as the control for loading. (f) The ratio of densitometry units of PUMA/β-actin is represented. The values are expressed as the means±S.D. (n=3 in each group) for western blotting