Table 3. Identified proteins by MS/MS analysis.

Spot no.	Protein description	Gene name	Function	Accession no.	Mra	Score	No. of pepb	Fold changec
1	Calpain small subunit 1	CAPNS1	Cell proliferation/differentiation	P04632	28 469	432	41	↓2.3±0.6
2	Rho GDP-dissociation inhibitor 1	ARHGDIA	Metabolism	P52565	23 250	135	12	↓3.6±0.5
3	D-3-phosphoglycerate dehydrogenase	PHGDH	Metabolism	O43175	57 356	94	17	↑2.1±0.3
4	60S acidic ribosomal protein P0	RPLP0	Protein binding	P05388	34 423	251	19	↓2.4±0.5
5	S-phase kinase-associated protein 1	SKP1	Proteolysis	P63208	18 817	205	11	↑2.2±0.2
6	Chloride intracellular channel protein 1	CLIC1	Ion channel	O00299	27 248	215	12	↓4.3±1.4
7	Proteasome subunit alpha type-3	PSMA3	Proteolysis	P25788	28 643	25	5	↓2.0±0.3
8	Pyruvate dehydrogenase E1 component subunit beta, mitochondrial	PDHB	Metabolism	P11177	39 550	231	13	↓2.3±0.4
9	Protein SEC13 homolog	SEC13	Protein binding	P55735	36 031	42	1	↓3.2±0.8
10	Eukaryotic translation initiation factor 3 subunit I	EIF3I	Transcription regulation	Q13347	36 878	86	15	↓5.6±1.3
11	Single-stranded DNA-binding protein, mitochondrial	SSBP1	DNA binding	Q04837	17 249	109	9	↑2.0±0.2
12	Eukaryotic translation initiation factor 4H	EIF4H	Transcription regulation	Q15056	27 425	70	3	↓NA
13	Histone H2B type 1-B	HIST1H2BB	DNA binding	P33778	13 942	288	11	↑NA
14	Protein DJ-1	PARK7	Protein binding	Q99497	20 050	166	10	↑3.7±1.3
15	Proteasome subunit alpha type-5	PSMA5	Proteolysis	P28066	26 565	103	11	↓3.3±0.7
16	Nucleoside diphosphate kinase B	NME2	DNA binding	P22392	17 401	124	29	↑3.1±1.2
17	Heterogeneous nuclear ribonucleoproteins A2/B1	HNRNPA2B1	mRNA splicing	P22626	37 464	197	17	↑2.2±0.6
18	Histone H2A type 2-A	H2A2A	Transcription regulation	Q6FI13	14 087	153	6	↓2.1±0.2
19	Retinal dehydrogenase 1	ALDH1A1	Metabolism	P01834	55 454	196	20	↑3.6±1.6
20	Lactoylglutathione lyase	GLO1	Zinc ion binding	Q04760	20 992	65	12	↓2.6±0.9
21	Ras-related protein Rab-7a	RAB7A	Signal transduction	P51149	23 760	46	2	↓2.0±0.3

All protein spots identified by ESI-Q-TOF MS/MS as significantly changed in average expression level in SKLB-163-treated A375 cells compared with the parental A375 cells

^aMr: Theoretical molecular weight (kDa) from the ExPASy database

^bNo. of pep: number of unique peptides identified by MS/MS sequencing

^cUpward arrows, upregulated; downward arrows, downregulated; NA, the spots on one of the paired gels were too weak or nondetectable