Figure 5.

Parkin miss function affects the amount of CED-10. (a) The strain ced-10(n3417) harboring the integrated array CED-10::GFP was crossed individually with the pdr-1 mutant strains, and the amount of GFP correlated with the amount of CED-10 detected by immunoblotting. Twenty micrograms of protein was electrophoresed and blotted against GFP (upper panel). The amount of CED-10::GFP was increased in both pdr-1 mutant strains analyzed. Antibody anti-β-actin was used as a loading control (bottom panel). The same results were obtained in three independent experiments. (b–d) Confocal fluorescent micrographs of CED-10 translational GFP reporter expression pattern and quantification of total CED-10::GFP in L4 animals. The most evident fluorescent organs are labeled by white arrows, vulva by light arrow and head by thick arrow. Scale bars in (b–d)=40 μm. (e) Values are means±s.e.m. (n=10–15 worms imaged in three independent experiments). **P<0.01 in the strain containing the allele pdr-1(lg103) compared with the control strain. P-value in the strain containing the allele pdr-1(tm395)=0.78948142