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. 2014 Jan 16;42(6):e47. doi: 10.1093/nar/gkt1363

Figure 1.

Figure 1.

Modified SSA assay measuring TALEN monomer activity in HEK293T cells. (A) Schematic of the PCR-based SSA assay. Target plasmids carrying the same left (L) and right (R) TALEN-target sequence were cleaved by a TALEN co-transfected into HEK293T cells. The SSA-repaired plasmid yielded a 345-bp PCR fragment which can be separated from the ∼514-bp PCR fragment amplified from unmodified or NHEJ-repaired plasmids. The stop sign marks the stop codon; GF and FP, flanking EGFP repeat regions; Z, GFP-ZFN target site; L, left TALEN target sequence; S, spacer; R, right TALEN target sequence. (B) A standard curve validating the modified SSA assay. Error bars, s.e.m. (n = 3).