Figure 2.

Validation of light-induction by whole-mount ISH and qRT-PCR. Upregulation of nine transcripts following 1-h of light (L) versus control darkness (D) is confirmed by quantitative whole-mount ISH on 48–72-h-old embryos (A–F) or by qRT-PCR on adult pineal gland samples (G–I). For ISH analysis, dorsal views of representative specimens are shown; signals in the pineal gland (A–C, green arrow) or the entire cranium area (D–F) were determined using ImageJ software. Error bars represent SE (A–F, n = 15–18; G–I, n = 3); *P < 0.05, **P < 0.01, ***P < 0.001, t-test. CT, circadian time.