Skip to main content
. 2014 Jan 13;42(6):3750–3767. doi: 10.1093/nar/gkt1359

Figure 8.

Figure 8.

miR-183 knockdown effects the pineal aanat2 mRNA rhythm. Zebrafish embryos were injected with control morpholino (cont MO) or miR-183 morpholino [miR-183(guide)MO] and entrained by two LD cycles. On the third day of development, embryos were transferred to DD, sampled at 4-h intervals and pineal aanat2 mRNA levels were determined by whole-mount ISH. Gray and black horizontal bars represent subjective day and night, respectively. Values represent the mean ± SE optical densities of the pineal signals (n = 15–20). Representative pineal aanat2 signals (dorsal view) of embryos treated with cont MO (top panel) and miR-183(guide)MO (bottom panel) from each group are presented. **P < 0.01, ***P < 0.001, t-test with Bonferroni correction. CT, circadian time.