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Biophysical characterization of human 80S ribosomes purified from HeLa cells. (A) 15–30% sucrose density gradient profiles for 80S, and dissociated 60S and 40S subunits depicting the separation of components based on density. (B) SEC-MALLS for 80S sample and (C) AUC results showing the homogeneity of 80S sample, which corresponds to the calculated molecular weight of 4.3 MDa.
