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. 2013 Dec 25;42(6):3954–3970. doi: 10.1093/nar/gkt1346

Figure 2.

Figure 2.

The CYT2 3′ UTR is sufficient to promote Puf3p-mediated mRNA decay, and the conserved UGUA element is required for Puf3p regulation. (A) CYT2 3′ UTR sequences immediately downstream of the translational stop codon for the WT MFA2/CYT2 and mutant MFA2/CYT2mut mRNAs. The conserved 10 nt Puf3p element is highlighted in bold text, with the UGUA element underlined in the WT transcript. In the mutant transcript, the UGUA element is mutated to ACAC, which is boxed. (B) Representative northern blots of the decay of MFA2, MFA2/CYT2 and MFA2/CYT2mut mRNAs expressed from a WT strain or a puf3Δ strain are shown. Minutes following transcriptional repression are indicated above each set of blots, with the half-lives (T1/2) and standard error of the mean (SEM) determined from multiple experiments.